DNA-catalyzed sequence-specific hydrolysis of DNA
نویسندگان
چکیده
منابع مشابه
DNA-catalyzed sequence-specific hydrolysis of DNA
Deoxyribozymes (DNA catalysts) have been reported for cleavage of RNA phosphodiester linkages, but cleaving peptide or DNA phosphodiester linkages is much more challenging. Using in vitro selection, here we identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and with a rate enhancement of 108 (possibly as high as 1014). The new DNA catalysts require both Mn...
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DNA catalysts (deoxyribozymes) for a variety of reactions have been identified by in vitro selection. However, for certain reactions this identification has not been achieved. One important example is DNA-catalyzed amide hydrolysis, for which a previous selection experiment instead led to DNA-catalyzed DNA phosphodiester hydrolysis. Subsequent efforts in which the selection strategy deliberatel...
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1. Shevach, E.M. Immunity 30, 636–645 (2009). 2. Yan, Z., Garg, S.K., Kipnis, J. & Banerjee, R. Nat. Chem. Biol. 5, 721–723 (2009). 3. Angelini, G. et al. Proc. Natl. Acad. Sci. USA 99, 1491–1496 (2002). 4. Castellani, P., Angelini, G., Delfino, L., Matucci, A. & Rubartelli, A. Eur. J. Immunol. 38, 2419–2425 (2008). 5. Kazama, H. et al. Immunity 29, 21–32 (2008). 6. Lim, S.Y., Raftery, M.J., Go...
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A three dimensional model of the basic Helix-Loop-Helix motif and its sequence specific recognition of DNA is described. The basic-helix I is modeled as a continuous ?-helix because no ?-helix breaking residue is found between the basic region and the first helix. When the basic region of the two peptide monomers are aligned in the successive major groove of the cognate DNA, the hydrophobi...
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We previously reported that DNA catalysts (deoxyribozymes) can hydrolyze DNA phosphodiester linkages, but DNA-catalyzed amide bond hydrolysis has been elusive. Here we used in vitro selection to identify DNA catalysts that hydrolyze ester linkages as well as DNA catalysts that hydrolyze aromatic amides, for which the leaving group is an aniline moiety. The aromatic amide-hydrolyzing deoxyribozy...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2009
ISSN: 1552-4450,1552-4469
DOI: 10.1038/nchembio.201